1. Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

(1).4 bp

(2). 10 bp

(3). 8 bp

(4). 6 bp

2. What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?
A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism.
B. It may get integrated into the genome of the recipient.
C. It may multiply and be inherited along with the host DNA.
D. The alien piece of DNA is not an integral part of chromosome.
E. It shows ability to replicate.
Choose the correct answer from the options given below :

(1).B and C only

(2). A and E only

(3). A and B only

(4). D and E only

3. The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes:

(1).The gene ' X ' is for protein involved in replication of Plasmid and ' Y ' for resistance to antibiotics .

(2). Gene ' X ' is responsible for recognition sites and ' Y ' is responsible for antibiotic resistance .

(3). The gene ' X ' is responsible for resistance to antibiotics and ' Y ' for protein involved in the replication of Plasmid .

(4). The gene ' X ' is responsible for controlling the copy number of the linked DNA and ' Y ' for protein involved in the replication of Plasmid .

4. The "Ti plasmid" of Agrobacterium tumefaciens stand for

(1).Tumor inducing plasmid

(2). Temperature independent plasmid

(3). Tumour inhibiting plasmid

(4). Tumor indepndent plasmid

5. Which of the following statements is incorrect?

(1).Bio - reactors are used to produce small scale bacterial cultures .

(2). Bio - reactors have an agitator system , an oxygen delivery system and foam control system .

(3). A bio - reactor provides optimal growth conditions for achieving the desired product .

(4). Most commonly used bio - reactors are of stirring type .

6. Given below are two statements :
Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below :

(1).Statement I is incorrect but statement Il is correct

(2). Both statement I and statement Il are correct

(3). Both statement I and statement II are incorrect

(4). Statement I is correct but statement II is incorrect

7. The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate.
Given below are two statements about this method:
Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies.
Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies.
In the light of the above statements, choose the most appropriate answer from the options given below :

(1).Statement I is incorrect but Statement II is correct

(2). Both Statement I and Statement II are correct

(3). Both Statement I and Statement II are incorrect

(4). Statement I is correct but Statement Il is incorrect

8. Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below :

(1).B and C only

(2). C and D only

(3). A and B only

(4). D and E only

9. Why can't insulin be given orally to diabetic patients?

(1).Its bioavailability will be increased

(2). Human body will elicit strong immune response

(3). It will be digested in Gastro-Intestinal (Gl) tract

(4). Because of structural variation

10. Silencing of specific mRNA is possible via RNAi because of -

(1).Non-complementary ssRNA

(2). Complementary dsRNA

(3). Inhibitory ssRNA

(4). Complementary tRNA

11. Identify the part of a bio-reactor which is used as a foam braker from the given figure.

(1).C

(2). A

(3). B

(4). D

12. Polymerase chain reaction (PCR) amplifies DNA following the equation.

(1).2N²

(2). N²

(3). 2ⁿ

(4). 2n+1

13. Which of the following genetically engineered organisms was used by Eli Lilly to prepare human insulin?

(1).Phage

(2). Bacterium

(3). Yeast

(4). Virus

14.
In the above represented plasmid an alien pieceof DNA is inserted af EcoRI site. Which of thefollowing strategies will be chosen to select therecombinant colonies?

(1).Blue color colonies grown on ampicillinplates can beselected.

(2). Using ampicillin & tetracyclin containingmedium plate.

(3). Blue color colonies will be selected.

(4). White color cofonies will be selected.