Year: 2024
Topic: Biotechnology : Principles and Processes
Time :
1. The following diagram showing restriction sites in
E.coli
cloning vector pBR322. Find the role of 'X' and 'Y' genes:
(1). The gene ' X ' is for protein involved in replication of Plasmid and ' Y ' for resistance to antibiotics .
(2). Gene ' X ' is responsible for recognition sites and ' Y ' is responsible for antibiotic resistance .
(3). The gene ' X ' is responsible for resistance to antibiotics and ' Y ' for protein involved in the replication of Plasmid .
(4). The gene ' X ' is responsible for controlling the copy number of the linked DNA and ' Y ' for protein involved in the replication of Plasmid .